Zebrafish (Danio rerio) ecotoxicological ABCB4, ABCC1 and ABCG2a gene promoters depict spatiotemporal xenobiotic multidrug resistance properties against environmental pollutants

Marine organisms are naturally equipped with multixenobiotic resistance mechanisms that often governed by ATP-binding cassette (ABC) transporter family members. Previous studies focused on the target genes of ABC but little is known about functionality their promoter regions. Due to importance promoters in gene regulation, we functionally characterized three major xenobiotic zebrafish, namely ABCB4, ABCC1 and ABCG2a via silico transcription factor binding analysis vivo spatiotemporal expression analysis. The former revealed functional contributors (such as AP-1, C/EBP beta, HNF-1 NF-1 TFBSs) towards activity enhancement across four different tissues (liver, muscle, cell cycle immune cells) where majority them discovered were liver-specific whereas latter unearthed localization these at liver intestinal tracts during late embryogenesis (48, 72 96 hpf). This study contributes future ecotoxicology zebrafish.